Skip to content
Open
Changes from all commits
Commits
File filter

Filter by extension

Filter by extension

Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
13 changes: 13 additions & 0 deletions genomeBrowserViz/README.md
Original file line number Diff line number Diff line change
@@ -0,0 +1,13 @@
## UCSC Genome Browser Visualizations
Track data hubs can be used to visualize genomics signal tracks using the UCSC Genome Browser (for more information, please see the [Raney et al., 2014](https://academic.oup.com/bioinformatics/article/30/7/1003/232409) publication.

We developed a track data hub to visualize maxATAC-derived _in-silico_ ChIP-seq datasets in tandem with chromatin accessibility data. Data can be explored in multiple ways using this tool.

### Visualization of Genomic Regions
After loading the track data hub (link [here](https://gb.research.cchmc.org/hub/group/memoryCD4/hub.txt)) in "My Data" > "Track Hubs" on the [UCSC Genome Browser website](https://genome.ucsc.edu/index.html), you can select specific genomic locations to visualize all subpopulation-resolved maxATAC predictions and chromatin accessibility information in that area. The image below shows the promoter and gene body of _IFNG_:

### Identification of Specific TFBS
Right-clicking on the maxATAC prediction track (as demonstrated below) expands the composite track such that all TFs with binding sites in that region can be visualized.

### Visualization of Specific Subpopulations
If you would like to visualize maxATAC predictions for a subset of subpopulations (e.g., the Th1 resting and active subpopulations), click on the... and select the desired subopoulations to visualize.