flow

Scripts for processing flow cytometry and FACS data

The overall strategy for using flow cytometry analysis is:

1. Put your .fcs files and a SampleSheet.txt in a folder.
2. Modify the script to point at that folder, or folders.
3. Run the script, gating when prompted. Modify the gating to gate on different parameters than those implemented.

./src/ contains snippets of R code for functions and things.

To run the example script, download the dataset from here. You can see the example of this analysis here.

We should have these pairs of example datasets and analyses, more of them.

• ~12 samples of dye concentration here.
• a plate of GAP1::GFP
• something else
• coulter counting

paging

To make it so that you can view HTML pages, go to repository settings and enable github pages and set it to read the master branch. Then put a link in here, if you want to. Note the relative links above work just fine.